NSFL1C

Protein-coding gene in the species Homo sapiens
NSFL1C
Available structures
PDBOrtholog search: PDBe RCSB
List of PDB id codes

1SS6

Identifiers
AliasesNSFL1C, P47, UBX1, UBXD10, UBXN2C, dJ776F14.1, NSFL1 cofactor
External IDsOMIM: 606610; MGI: 3042273; HomoloGene: 41114; GeneCards: NSFL1C; OMA:NSFL1C - orthologs
Gene location (Human)
Chromosome 20 (human)
Chr.Chromosome 20 (human)[1]
Chromosome 20 (human)
Genomic location for NSFL1C
Genomic location for NSFL1C
Band20p13Start1,442,162 bp[1]
End1,473,842 bp[1]
Gene location (Mouse)
Chromosome 2 (mouse)
Chr.Chromosome 2 (mouse)[2]
Chromosome 2 (mouse)
Genomic location for NSFL1C
Genomic location for NSFL1C
Band2|2 G3Start151,336,102 bp[2]
End151,353,334 bp[2]
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • right adrenal gland

  • right adrenal cortex

  • left adrenal gland

  • gastrocnemius muscle

  • left adrenal cortex

  • skin of leg

  • tibialis anterior muscle

  • skin of abdomen

  • retinal pigment epithelium

  • monocyte
Top expressed in
  • tail of embryo

  • lens

  • neural layer of retina

  • genital tubercle

  • neural tube

  • hypothalamus

  • yolk sac

  • olfactory bulb

  • ventricular zone

  • ovary
More reference expression data
BioGPS




More reference expression data
Gene ontology
Molecular function
  • lipid binding
  • ubiquitin binding
  • protein binding
  • phospholipid binding
  • protein phosphatase regulator activity
  • ATPase binding
Cellular component
  • Golgi stack
  • plasma membrane
  • Golgi apparatus
  • intermediate filament cytoskeleton
  • VCP-NSFL1C complex
  • nucleus
  • nucleoplasm
  • chromosome
  • cytosol
  • spindle pole centrosome
  • cytoplasm
  • microtubule organizing center
  • cytoskeleton
Biological process
  • membrane fusion
  • nuclear membrane reassembly
  • Golgi organization
  • proteasome-mediated ubiquitin-dependent protein catabolic process
  • autophagosome assembly
  • regulation of phosphoprotein phosphatase activity
  • establishment of mitotic spindle orientation
  • positive regulation of mitotic centrosome separation
  • negative regulation of protein localization to centrosome
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

55968

386649

Ensembl

ENSG00000088833

ENSMUSG00000027455

UniProt

Q9UNZ2

Q9CZ44

RefSeq (mRNA)

NM_001206736
NM_016143
NM_018839
NM_182483

NM_001291074
NM_198326

RefSeq (protein)

NP_001193665
NP_057227
NP_061327

NP_001278003
NP_938085

Location (UCSC)Chr 20: 1.44 – 1.47 MbChr 2: 151.34 – 151.35 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

NSFL1 cofactor p47 is a protein that in humans is encoded by the NSFL1C gene.[5][6]

N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Multiple transcript variants encoding several different isoforms have been found for this gene.[6]

Interactions

NSFL1C has been shown to interact with Valosin-containing protein.[7]

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000088833 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000027455 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Zhang QH, Ye M, Wu XY, Ren SX, Zhao M, Zhao CJ, Fu G, Shen Y, Fan HY, Lu G, Zhong M, Xu XR, Han ZG, Zhang JW, Tao J, Huang QH, Zhou J, Hu GX, Gu J, Chen SJ, Chen Z (Nov 2000). "Cloning and Functional Analysis of cDNAs with Open Reading Frames for 300 Previously Undefined Genes Expressed in CD34+ Hematopoietic Stem/Progenitor Cells". Genome Res. 10 (10): 1546–60. doi:10.1101/gr.140200. PMC 310934. PMID 11042152.
  6. ^ a b "Entrez Gene: NSFL1C NSFL1 (p97) cofactor (p47)".
  7. ^ Uchiyama, Keiji; Jokitalo Eija; Lindman Mervi; Jackman Mark; Kano Fumi; Murata Masayuki; Zhang Xiaodong; Kondo Hisao (Jun 2003). "The localization and phosphorylation of p47 are important for Golgi disassembly–assembly during the cell cycle". J. Cell Biol. 161 (6). United States: 1067–79. doi:10.1083/jcb.200303048. ISSN 0021-9525. PMC 2173005. PMID 12810701.

Further reading

  • Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
  • Yu W, Andersson B, Worley KC, et al. (1997). "Large-Scale Concatenation cDNA Sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
  • Kondo H, Rabouille C, Newman R, et al. (1997). "p47 is a cofactor for p97-mediated membrane fusion". Nature. 388 (6637): 75–8. Bibcode:1997Natur.388R..75K. doi:10.1038/40411. PMID 9214505. S2CID 32646222.
  • Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
  • Rabouille C, Kondo H, Newman R, et al. (1998). "Syntaxin 5 is a common component of the NSF- and p97-mediated reassembly pathways of Golgi cisternae from mitotic Golgi fragments in vitro". Cell. 92 (5): 603–10. doi:10.1016/S0092-8674(00)81128-9. PMID 9506515. S2CID 17285800.
  • Hu RM, Han ZG, Song HD, et al. (2000). "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning". Proc. Natl. Acad. Sci. U.S.A. 97 (17): 9543–8. Bibcode:2000PNAS...97.9543H. doi:10.1073/pnas.160270997. PMC 16901. PMID 10931946.
  • Deloukas P, Matthews LH, Ashurst J, et al. (2002). "The DNA sequence and comparative analysis of human chromosome 20". Nature. 414 (6866): 865–71. Bibcode:2001Natur.414..865D. doi:10.1038/414865a. PMID 11780052.
  • Meyer HH, Wang Y, Warren G (2002). "Direct binding of ubiquitin conjugates by the mammalian p97 adaptor complexes, p47 and Ufd1–Npl4". EMBO J. 21 (21): 5645–52. doi:10.1093/emboj/cdf579. PMC 131076. PMID 12411482.
  • Uchiyama K, Jokitalo E, Kano F, et al. (2003). "VCIP135, a novel essential factor for p97/p47-mediated membrane fusion, is required for Golgi and ER assembly in vivo". J. Cell Biol. 159 (5): 855–66. doi:10.1083/jcb.200208112. PMC 2173386. PMID 12473691.
  • Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
  • Nagahama M, Suzuki M, Hamada Y, et al. (2003). "SVIP Is a Novel VCP/p97-interacting Protein Whose Expression Causes Cell Vacuolation". Mol. Biol. Cell. 14 (1): 262–73. doi:10.1091/mbc.02-07-0115. PMC 140243. PMID 12529442.
  • Uchiyama K, Jokitalo E, Lindman M, et al. (2003). "The localization and phosphorylation of p47 are important for Golgi disassembly–assembly during the cell cycle". J. Cell Biol. 161 (6): 1067–79. doi:10.1083/jcb.200303048. PMC 2173005. PMID 12810701.
  • Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
  • Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
  • Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
  • Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
  • Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
  • v
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  • 1i42: NMR STRUCTURE OF THE UBX DOMAIN FROM P47
    1i42: NMR STRUCTURE OF THE UBX DOMAIN FROM P47
  • 1jru: NMR STRUCTURE OF THE UBX DOMAIN FROM P47 (ENERGY MINIMISED AVERAGE)
    1jru: NMR STRUCTURE OF THE UBX DOMAIN FROM P47 (ENERGY MINIMISED AVERAGE)
  • 1s3s: Crystal structure of AAA ATPase p97/VCP ND1 in complex with p47 C
    1s3s: Crystal structure of AAA ATPase p97/VCP ND1 in complex with p47 C
  • 1ss6: Solution structure of SEP domain from human p47
    1ss6: Solution structure of SEP domain from human p47
  • 1v92: Solution structure of the UBA domain from p47, a major cofactor of the AAA ATPase p97
    1v92: Solution structure of the UBA domain from p47, a major cofactor of the AAA ATPase p97
  • 1vaz: Solution structures of the p47 SEP domain
    1vaz: Solution structures of the p47 SEP domain