SF3A2

Protein-coding gene in the species Homo sapiens

SF3A2

Identifiers

Aliases

SF3A2, PRP11, PRPF11, SAP62, SF3a66, splicing factor 3a subunit 2

External IDs

OMIM: 600796; MGI: 104912; HomoloGene: 136815; GeneCards: SF3A2; OMA:SF3A2 - orthologs

Gene location (Human)

Chr.	Chromosome 19 (human)^[1]

Band	19p13.3	Start	2,236,824 bp^[1]
Band	19p13.3	End	2,248,655 bp^[1]

Gene location (Mouse)

Chr.	Chromosome 10 (mouse)^[2]

Band	10 C1\|10 39.72 cM	Start	80,634,032 bp^[2]
Band	10 C1\|10 39.72 cM	End	80,640,758 bp^[2]

RNA expression pattern

Bgee

Human

Mouse (ortholog)

Top expressed in

left testis

left ovary

right testis

right uterine tube

right ovary

body of uterus

granulocyte

canal of the cervix

skin of leg

skin of abdomen

Top expressed in

internal carotid artery

external carotid artery

Ileal epithelium

Paneth cell

lactiferous gland

condyle

somite

trigeminal ganglion

fossa

hair follicle

More reference expression data

BioGPS


More reference expression data

Gene ontology
Molecular function	nucleic acid binding zinc ion binding protein binding metal ion binding RNA binding
Cellular component	nuclear speck catalytic step 2 spliceosome spliceosomal complex small nuclear ribonucleoprotein complex U2-type prespliceosome nucleus U2 snRNP nucleoplasm U2-type precatalytic spliceosome
Biological process	mRNA splicing, via spliceosome mRNA processing mRNA 3'-splice site recognition RNA splicing positive regulation of neuron projection development spliceosomal complex assembly U2-type prespliceosome assembly
Sources:Amigo / QuickGO

Orthologs

Species

Human

Mouse

Entrez


8175


20222

Ensembl


ENSG00000104897


ENSMUSG00000020211

UniProt


Q15428


Q62203

RefSeq (mRNA)


NM_007165


NM_013651

RefSeq (protein)


NP_009096


n/a

Location (UCSC)

Chr 19: 2.24 – 2.25 Mb

Chr 10: 80.63 – 80.64 Mb

PubMed search

^[3]

^[4]

Wikidata

View/Edit Human

View/Edit Mouse

Splicing factor 3A subunit 2 is a protein that in humans is encoded by the SF3A2 gene.^[5]^[6]^[7]

Function

This gene encodes subunit 2 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 2 interacts with subunit 1 through its amino-terminus while the single zinc finger domain of subunit 2 plays a role in its binding to the 15S U2 snRNP. Subunit 2 may also function independently of its RNA splicing function as a microtubule-binding protein.^[7]

Interactions

SF3A2 has been shown to interact with DDX46.^[8]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000104897 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000020211 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Bennett M, Reed R (October 1993). "Correspondence between a mammalian spliceosome component and an essential yeast splicing factor". Science. 262 (5130): 105–8. Bibcode:1993Sci...262..105B. doi:10.1126/science.8211113. PMID 8211113.
^ Dresser DW, Hacker A, Lovell-Badge R, Guerrier D (September 1995). "The genes for a spliceosome protein (SAP62) and the anti-Müllerian hormone (AMH) are contiguous". Human Molecular Genetics. 4 (9): 1613–8. doi:10.1093/hmg/4.9.1613. PMID 8541848.
^ ^a ^b "Entrez Gene: SF3A2 splicing factor 3a, subunit 2, 66kDa".
^ Will CL, Urlaub H, Achsel T, Gentzel M, Wilm M, Lührmann R (September 2002). "Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein". The EMBO Journal. 21 (18): 4978–88. doi:10.1093/emboj/cdf480. PMC 126279. PMID 12234937.

Chiara MD, Champion-Arnaud P, Buvoli M, Nadal-Ginard B, Reed R (July 1994). "Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114". Proceedings of the National Academy of Sciences of the United States of America. 91 (14): 6403–7. Bibcode:1994PNAS...91.6403C. doi:10.1073/pnas.91.14.6403. PMC 44210. PMID 8022796.
Rain JC, Tartakoff AM, Krämer A, Legrain P (June 1996). "Essential domains of the PRP21 splicing factor are implicated in the binding to PRP9 and PRP11 proteins and are conserved through evolution". RNA. 2 (6): 535–50. PMC 1369393. PMID 8718683.
Hong W, Bennett M, Xiao Y, Feld Kramer R, Wang C, Reed R (January 1997). "Association of U2 snRNP with the spliceosomal complex E". Nucleic Acids Research. 25 (2): 354–61. doi:10.1093/nar/25.2.354. PMC 146436. PMID 9016565.
Moraitou M, Patrinou-Georgoula M, Guialis A (May 1998). "Structural/functional properties of a mammalian multi-component structure containing all major spliceosomal small nuclear ribonucleoprotein particles". The Biochemical Journal. 332 (1): 135–44. doi:10.1042/bj3320135. PMC 1219461. PMID 9576861.
Yan D, Perriman R, Igel H, Howe KJ, Neville M, Ares M (September 1998). "CUS2, a yeast homolog of human Tat-SF1, rescues function of misfolded U2 through an unusual RNA recognition motif". Molecular and Cellular Biology. 18 (9): 5000–9. doi:10.1128/mcb.18.9.5000. PMC 109085. PMID 9710584.
Neubauer G, King A, Rappsilber J, Calvio C, Watson M, Ajuh P, Sleeman J, Lamond A, Mann M (September 1998). "Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex". Nature Genetics. 20 (1): 46–50. doi:10.1038/1700. PMID 9731529. S2CID 585778.
Krämer A, Grüter P, Gröning K, Kastner B (June 1999). "Combined biochemical and electron microscopic analyses reveal the architecture of the mammalian U2 snRNP". The Journal of Cell Biology. 145 (7): 1355–68. doi:10.1083/jcb.145.7.1355. PMC 2133165. PMID 10385517.
Das R, Zhou Z, Reed R (May 2000). "Functional association of U2 snRNP with the ATP-independent spliceosomal complex E". Molecular Cell. 5 (5): 779–87. doi:10.1016/S1097-2765(00)80318-4. PMID 10882114.
Will CL, Schneider C, MacMillan AM, Katopodis NF, Neubauer G, Wilm M, Lührmann R, Query CC (August 2001). "A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site". The EMBO Journal. 20 (16): 4536–46. doi:10.1093/emboj/20.16.4536. PMC 125580. PMID 11500380.
Nesic D, Krämer A (October 2001). "Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation". Molecular and Cellular Biology. 21 (19): 6406–17. doi:10.1128/MCB.21.19.6406-6417.2001. PMC 99788. PMID 11533230.
Dresser DW, Jamin SP, Atkins CJ, Guerrier D (October 2001). "An expressed GNRP-like gene shares a bi-directional promoter with SF3A2 (SAP62) immediately upstream of AMH". Gene. 277 (1–2): 163–73. doi:10.1016/S0378-1119(01)00690-4. PMID 11602354.
Jurica MS, Licklider LJ, Gygi SR, Grigorieff N, Moore MJ (April 2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis". RNA. 8 (4): 426–39. doi:10.1017/S1355838202021088. PMC 1370266. PMID 11991638.
Will CL, Urlaub H, Achsel T, Gentzel M, Wilm M, Lührmann R (September 2002). "Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein". The EMBO Journal. 21 (18): 4978–88. doi:10.1093/emboj/cdf480. PMC 126279. PMID 12234937.
Takenaka K, Nakagawa H, Miyamoto S, Miki H (August 2004). "The pre-mRNA-splicing factor SF3a66 functions as a microtubule-binding and -bundling protein". The Biochemical Journal. 382 (Pt 1): 223–30. doi:10.1042/BJ20040521. PMC 1133934. PMID 15142036.
Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik J, Legrain P, Gauthier JM (July 2004). "Functional proteomics mapping of a human signaling pathway". Genome Research. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC 442148. PMID 15231748.
Nesic D, Tanackovic G, Krämer A (September 2004). "A role for Cajal bodies in the final steps of U2 snRNP biogenesis". Journal of Cell Science. 117 (Pt 19): 4423–33. doi:10.1242/jcs.01308. PMID 15316075.