Fitoen desaturaza (formira likopen)
| Fitoen desaturaza (formira likopen) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.3.99.31 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Fitoen desaturaza (formira likopen) (EC 1.3.99.31, četvorostepna fitoenska desaturaza, fitoenska desaturaza (nespecifična), CrtI (nespecifična)) je enzim sa sistematskim imenom 15-cis-fitoen:akceptor oksidoreduktaza (formira likopen).[1] Ovaj enzim katalizuje sledeću hemijsku reakciju
- 15-cis-fitoen + 4 akceptor sve-trans-likopen + 4 redukovani akceptor (sveukupna reakcija)
- (1a) 15-cis-fitoen + akceptor sve-trans-fitofluen + redukovani akceptor
- (1b) sve-trans-fitofluen + akceptor sve-trans-zeta-karoten + redukovani akceptor
- (1c) sve-trans-zeta-karoten + akceptor sve-trans-neurosporen + redukovani akceptor
- (1d) sve-trans-neurosporen + akceptor sve-trans-likopen + redukovani akceptor
Za rad ovog enzima je neophodan FAD. On učestvuje u biosintezi karotenoida i katalizuje do četiri koraka desaturacije, cf. EC 1.3.99.28, fitoenska desaturaza (formira neurosporen), EC 1.3.99.29, fitoenska desaturaza (formira zeta-karoten) i EC 1.3.99.30, fitoenska desaturaza (formira 3,4-didehidrolikopen).
Reference
- ↑ Fraser, P.D., Misawa, N., Linden, H., Yamano, S., Kobayashi, K. and Sandmann, G. (1992). „Expression in Escherichia coli, purification, and reactivation of the recombinant Erwinia uredovora phytoene desaturase”. J. Biol. Chem. 267: 19891-19895. PMID 1400305.
Literatura
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.
Spoljašnje veze
- MeSH Phytoene+desaturase+(lycopene-forming)
- p
- r
- u
Aktivno mesto • Alosterna regulacija • Mesto vezivanja • Katalitički perfektan enzim • Koenzim • Kofaktor • Kooperativnost • EC broj • Enzimska kataliza • Inhibicija enzima • Enzimska kinetika • Lajnviver–Burk dijagram • Mihaelis–Mentenova kinetika • Spisak enzima
EC1 Oksidoreduktaze/spisak • EC2 Transferaze/spisak • EC3 Hidrolaze/spisak • EC4 Lijaze/spisak • EC5 Izomeraze/spisak • EC6 Ligaze/spisak
B enzm: 1.1/2/3/4/5/6/7/8/10/11/13/14/15-18, 2.1/2/3/4/5/6/7/8, 2.7.10, 2.7.11-12, 3.1/2/3/4/5/6/7, 3.1.3.48, 3.4.21/22/23/24, 4.1/2/3/4/5/6, 5.1/2/3/4/99, 6.1-3/4/5-6
