RAP1A

RAP1A, član RAS familije onkogena
PDB prikaz baziran na 1c1y​.
Dostupne strukture
1C1Y​, 1GUA​, 3KUC​
Identifikatori
Simboli RAP1A; KREV-1; KREV1; RAP1; SMGP21
Vanjski ID OMIM: 179520 MGI: 97852 HomoloGene: 2162 GeneCards: RAP1A Gene
Ontologija gena
Molekularna funkcija • vezivanje nukleotida
• aktivnost GTPaze
• proteinsko vezivanje
• GTP vezivanje
• vezivanje Ras GTPaze
Celularna komponenta • citozol
• ćelijska membrana
• kompleks faktora razmene guanil nukleotida
Biološki proces • aktivacija MAPKK aktivnosti
• metabolički proces energijske rezerve
• GTP katabolički proces
• prenos signala
• prenos signala posredovan malim GTPazama
• koagulacija krvi
• aktivacija trombocita
• metabolički proces malih molekula
• signalni put receptora faktora nervnog rasta
• regulacija sekrecije insulina
Ortolozi
Vrsta Čovek Miš
Entrez 5906 109905
Ensembl ENSG00000116473 ENSMUSG00000068798
UniProt P62834 P62835
RefSeq (mRNA) NM_001010935.1 NM_145541.4
RefSeq (protein) NP_001010935.1 NP_663516.1
Lokacija (UCSC) Chr 1:
112.08 - 112.26 Mb		 Chr 3:
105.53 - 105.6 Mb
PubMed pretraga [1] [2]

Ras srodni protein 1A je protein koji je kod ljudi kodiran RAP1A genom.[1]

Interakcije

RAP1A formira interakcije sa C-Raf,[2][3][4][5] PDE6D,[6][7] TSC2,[8][9] RALGDS,[10][11] RAPGEF2[12] i MLLT4.[10]

Reference

  1. ^ Kawata M, Matsui Y, Kondo J, Hishida T, Teranishi Y, Takai Y (1989). „A novel small molecular weight GTP-binding protein with the same putative effector domain as the ras proteins in bovine brain membranes. Purification, determination of primary structure, and characterization”. J Biol Chem. 263 (35): 18965—71. PMID 3143720. 
  2. ^ Han, L; Colicelli J (1995). „A human protein selected for interference with Ras function interacts directly with Ras and competes with Raf1”. Mol. Cell. Biol. UNITED STATES. 15 (3): 1318—23. ISSN 0270-7306. PMC 230355 Слободан приступ. PMID 7862125. 
  3. ^ Nassar, N; Horn G, Herrmann C; et al. (1995). „The 2.2 A crystal structure of the Ras-binding domain of the serine/threonine kinase c-Raf1 in complex with Rap1A and a GTP analogue”. Nature. ENGLAND. 375 (6532): 554—60. ISSN 0028-0836. PMID 7791872. doi:10.1038/375554a0. 
  4. ^ Hu, C D; Kariya K; et al. (1999). „Effect of phosphorylation on activities of Rap1A to interact with Raf-1 and to suppress Ras-dependent Raf-1 activation”. J. Biol. Chem. UNITED STATES. 274 (1): 48—51. ISSN 0021-9258. PMID 9867809. doi:10.1074/jbc.274.1.48. 
  5. ^ Okada, T; Hu C D; et al. (1999). „The strength of interaction at the Raf cysteine-rich domain is a critical determinant of response of Raf to Ras family small GTPases”. Mol. Cell. Biol. UNITED STATES. 19 (9): 6057—64. ISSN 0270-7306. PMC 84512 Слободан приступ. PMID 10454553. 
  6. ^ Nancy, Vanessa; Callebaut Isabelle; et al. (2002). „The delta subunit of retinal rod cGMP phosphodiesterase regulates the membrane association of Ras and Rap GTPases”. J. Biol. Chem. United States. 277 (17): 15076—84. ISSN 0021-9258. PMID 11786539. doi:10.1074/jbc.M109983200. 
  7. ^ Hanzal-Bayer, Michael; Renault Louis; et al. (2002). „The complex of Arl2-GTP and PDE delta: from structure to function”. EMBO J. England. 21 (9): 2095—106. ISSN 0261-4189. PMC 125981 Слободан приступ. PMID 11980706. doi:10.1093/emboj/21.9.2095. 
  8. ^ Castro, Ariel F; Rebhun John F; et al. (2003). „Rheb binds tuberous sclerosis complex 2 (TSC2) and promotes S6 kinase activation in a rapamycin- and farnesylation-dependent manner”. J. Biol. Chem. United States. 278 (35): 32493—6. ISSN 0021-9258. PMID 12842888. doi:10.1074/jbc.C300226200. 
  9. ^ Yamamoto, Yuji; Jones Kathryn A; et al. (2002). „Multicompartmental distribution of the tuberous sclerosis gene products, hamartin and tuberin”. Arch. Biochem. Biophys. United States. 404 (2): 210—7. ISSN 0003-9861. PMID 12147258. doi:10.1016/S0003-9861(02)00300-4. 
  10. ^ а б Boettner, B; Govek E E; et al. (2000). „The junctional multidomain protein AF-6 is a binding partner of the Rap1A GTPase and associates with the actin cytoskeletal regulator profilin”. Proc. Natl. Acad. Sci. U.S.A. UNITED STATES. 97 (16): 9064—9. ISSN 0027-8424. PMC 16822 Слободан приступ. PMID 10922060. doi:10.1073/pnas.97.16.9064. 
  11. ^ Nancy, V; Wolthuis R M; et al. (1999). „Identification and characterization of potential effector molecules of the Ras-related GTPase Rap2”. J. Biol. Chem. UNITED STATES. 274 (13): 8737—45. ISSN 0021-9258. PMID 10085114. doi:10.1074/jbc.274.13.8737. 
  12. ^ Rebhun, J F; Castro A F; Quilliam L A (2000). „Identification of guanine nucleotide exchange factors (GEFs) for the Rap1 GTPase. Regulation of MR-GEF by M-Ras-GTP interaction”. J. Biol. Chem. UNITED STATES. 275 (45): 34901—8. ISSN 0021-9258. PMID 10934204. doi:10.1074/jbc.M005327200. 
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PDB Galerija
  • 1c1y​: Kristalna struktura RAP.GMPPNP u kompleksu sa Ras vezujućim domenom C-Raf1 kinaze (RAFRBD).
    1c1y​: Kristalna struktura RAP.GMPPNP u kompleksu sa Ras vezujućim domenom C-Raf1 kinaze (RAFRBD).
  • 1gua​: Ljudski RAP1A, ostaci 1-167, dvostruki mutant (E30D,K31E) u kompleksu sa GPPNHP i RAS vezujućim domenom ljudskog C-RAF1, ostaci 51-131
    1gua​: Ljudski RAP1A, ostaci 1-167, dvostruki mutant (E30D,K31E) u kompleksu sa GPPNHP i RAS vezujućim domenom ljudskog C-RAF1, ostaci 51-131